Innovation in detection of microparticles and exosomes
||E. van der Pol, F. Coumans, Z. Varga, M. Krumrey, and R. Nieuwland|
||Published June 30, 2013|
||Journal of Thrombosis and Haemostasis|
||van der Pol 2013 JTH Innovation in detection.pdf (539 kB)|
Cell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body ﬂuids such as blood. Although such vesicles have gained strong clinical and scientiﬁc interest, their detection is difﬁcult because many vesicles are extremely small with a diameter of less than 100 nm, and, moreover, these vesicles have a low refractive index and are heterogeneous in both size and composition. In this review, we focus on the relatively high throughput detection of vesicles in suspension by ﬂow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and we will discuss their applicability and limitations. Finally, we discuss four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small-angle X-ray scattering (SAXS), and anomalous SAXS. These methods are currently being explored to study vesicles and are likely to offer novel information for future developments.