Towards traceable size determination of extracellular vesicles
||Z. Varga, Y. Yuana, A.E. Grootemaat, E. van der Pol, C. Gollwitzer, M. Krumrey, R. Nieuwland|
||Published February 4, 2014|
||Journal of Extracellular Vesicles|
||Varga 2014 JEV Traceable size determination.pdf (1,554 kB)|
Extracellular vesicles (EVs) have clinical importance due to their roles in a wide range of biological processes. The detection and characterization of EVs are challenging because of their small size, low refractive index, and heterogeneity. In this manuscript, the size distribution of an erythrocyte-derived EV sample is determined using state-of-the-art techniques such as nanoparticle tracking analysis, resistive pulse sensing, and electron microscopy, and novel techniques in the field, such as small-angle X-ray scattering (SAXS) and size exclusion chromatography coupled with dynamic light scattering detection. The mode values of the size distributions of the studied erythrocyte EVs reported by the different methods show only small deviations, but there are differences in the widths of the size distributions. All the methods are compared and discussed in this manuscript with respect to traceability. Particularly, SAXS is promising technique in this regard, as this technique was already applied for traceable size determination of solid nanoparticles in suspension. To reach the traceable measurement of EVs, monodisperse and highly concentrated samples are required.