Detection and isolation of intact extracellular vesicles

Content

• Detection and isolation of intact extracellular vesicles by Rienk Nieuwland and Edwin van der Pol

1. Extracellular vesicle essentials by Edwin van der Pol and Rienk Nieuwland
2. Detection
1. Multiple particle detection
1. Surface
• Bead-capture flow cytometry by André Görgens and Joshua Welsh
• Enzyme-linked immunoassay (vacant)
• Surface plasmon resonance by Randy Carney and Tatu Rojalin
2. Suspension
• Analytical centrifugation (vacant)
• Dynamic light scattering (vacant)
• Multi-angle light scattering (vacant)
2. Single particle detection
1. Microscopy
• Atomic force microscopy by Daan Vorselen
• Electron microscopy by Alain Brisson, Veronika Kralj-Iglic and Yeshayahu Talmon
• Optical microscopy
• Fluorescence by Charles Lai
• Single particle interferometric reflectance imaging sensor by Olesia Gololobova and Kenneth Witwer
• Single molecule localization microscopy by Tijana Jovanovic-Talisman
2. Suspension
• Flow cytometry by Joanne Lannigan
• Nanoparticle tracking analysis by Edwin van der Pol
• Resistive pulse sensing by Edwin van der Pol
3. Isolation
1. Multiple particle isolation
1. Surface
• Affinity capture by Zoltán Wiener
2. Suspension
• Centrifugation
• Density gradient centrifugation by An Hendrix
• Ultracentrifugation by Willem Stoorvogel
• Field flow fractionation by Wyatt Vreeland
• Ultrafiltration by Joel Nordin
• Precipitation by Tong Wang
• Size-exclusion chromatography by Jan van Deun
2. Single particle isolation
• Flow cytometry sorting by Joshua Welsh and Jennifer Jones
4. Future developments
• Upcoming
• Standardization by Britta Bettin
• Metrology by Zoltán Varga
• Detection
• Fluorescence correlation spectroscopy by Wolf Heusermann and Nicole Meisner-Kober
• Raman spectroscopy by Agustin Enciso-Martinez, Tatu Rojalin and Randy Carney
• Isolation
• Cellulose nanofiber-based sheets by Akira Yokoi and Takao Yasui