Detection and isolation of intact extracellular vesicles

“Detection and isolation of intact extracellular vesicles (EVs)” is the third massive online open course (MOOC) of the International Society for Extracellular Vesicles (ISEV) organized by the Educational Committee under supervision of Rienk Nieuwland and myself.


  1. Extracellular vesicle essentials by Edwin van der Pol and Rienk Nieuwland (expected in June)
  2. Detection
    1. Multiple particle detection
      1. Surface
      2. Suspension
        • Analytical centrifugation (vacant)
        • Dynamic light scattering (vacant)
        • Multi-angle light scattering (vacant)
    2. Single particle detection
      1. Microscopy
        • Atomic force microscopy by Daan Vorselen
        • Electron microscopy by Alain Brisson, Veronika Kralj-Iglic and Yeshayahu Talmon (expected in June)
        • Optical microscopy
          • Fluorescence by Charles Lai (expected in June)
          • Single particle interferometric reflectance imaging sensor by Olesia Gololobova and Kenneth Witwer (expected in June)
          • Single molecule localization microscopy by Tijana Jovanovic-Talisman
      2. Suspension
        • Flow cytometry by Joanne Lannigan (expected in June)
        • Nanoparticle tracking analysis by Edwin van der Pol (expected in June)
        • Resistive pulse sensing by Edwin van der Pol (expected in June)
  3. Isolation
    1. Multiple particle isolation
      1. Surface
      2. Suspension
    2. Single particle isolation
      • Flow cytometry sorting by Joshua Welsh and Jennifer Jones (expected in June)
  4. Future developments