Presentations

The list below contains presentations which I presented.

2018

41 E. van der Pol
Detection of extracellular vesicles: size does matter (pdf, 3,508 kB)
Genootschap ter bevordering van Natuur-, Genees- en Heelkunde
November 16, 2018, Amsterdam, The Netherlands. Awarded
40 E. van der Pol
Detection of extracellular vesicles by flow cytometry: size does matter (pdf, 7,152 kB)
Brazil ISAC Flow cytometry workshop
November 6, 2018, São Paulo, Brazil. Invited

2017

39 E. van der Pol
Counting biological nanoparticles as biomarker for disease
Precisiebeurs 2017
November 16th, 2017, Veldhoven, The Netherlands. Invited
38 E. van der Pol
Detecting cell-derived vesicles with cell counters: from artefact to clinical act
European Society for Clinical Cell Analysis 2017
September 25th, 2017, Thessaloniki, Greece. Invited
37 E. van der Pol
Measuring extracellular vesicle concentrations: standardize the unknown
Scientific and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis
July 8th, 2017, Berlin, Germany. Invited
36 E. van der Pol
Detection of extracellular vesicles as biomarker for cancer
Cancer Center Amsterdam Seminar Series
May 11, 2017, Amsterdam, The Netherlands. Invited

2016

35 E. van der Pol, F.A.W. Coumans, A. Sturk, R. Nieuwland, S. Weidlich, Y. Lahini, M.A. Schmidt, S. Faez, and T.G. van Leeuwen
Refractive index and size determination of extracellular vesicles in a nano-fluidic optical fiber
International MicroNanoConference 2016
December 14, 2016, Amsterdam, The Netherlands
34 E. van der Pol, F.A. Coumans, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Flow cytometry standardization by size and refractive index determination
Mini-Symposium on characterization of extracellular vesicles by flow cytometry, VU University Medical Center
November 11, 2016, Amsterdam, The Netherlands. Invited
33 E. van der Pol, F.A. Coumans, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Single extracellular vesicle analysis: nanoparticle tracking analysis, tunable resistive pulse sensing, and scatter-based flow cytometry
26th Cytometry Development Workshop
October 26, 2016, San Diego, United States. Invited
32 E. van der Pol
The pathway to single vesicle detection
Gordon Research Conference on Extracellular Vesicles 2016
August 20, 2016, Sunday River, Maine, United States. Keynote
31 E. van der Pol, F.A. Coumans, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Standardisation of extracellular vesicle measurements
SelectBio Extracellular vesicles 2016
July 12, 2016, Cambridge, United Kingdom. Invited
30 E. van der Pol, F.A. Coumans, L. de Rond, E.L. de Gool, A.N. Böing, A. Sturk, R. Nieuwland, and T.G. van Leeuwen
Size and refractive index determination of submicrometer particles by flow cytometry
31st Congress of the International Society for Advancement of Cytometry
June 13, 2016, Seattle, United States
29 E. van der Pol, F.A. Coumans, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Quantitative light scattering to standardize flow cytometry measurements of extracellular vesicles
Scientific and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis
May 27, 2016, Montpellier, France. Invited
28 E. van der Pol, F.A. Coumans, S. Weidlich, Y. Lahini, M.A. Schmidt, T.G. van Leeuwen, A. Sturk, R. Nieuwland, and S. Faez
Size and refractive index determination of extracellular vesicles smaller than 70 nm in a nano-fluidic optical fiber
International Society for Extracellular Vesicles 2016
May 6, 2016, Rotterdam, The Netherlands
27 E. van der Pol, D.E. Every, F.A. Coumans, T.G. van Leeuwen, A. Sturk, and R. Nieuwland
Tunable resistive pulse sensing analysis of extracellular vesicles
International Society for Extracellular Vesicles 2016
May 3, 2016, Rotterdam, The Netherlands. Invited
pdf (1,168 kB)
26 E. van der Pol, S. Weidlich, Y. Lahini, F.A.W. Coumans, A. Sturk, R. Nieuwland, Markus A. Schmidt, S. Faez, and T.G. van Leeuwen
Label-free tracking of single extracellular vesicles in a nano-fluidic optical fiber
SPIE Photonics West 2016
February 13, 2016, San Francisco, United States

2015

25 E. van der Pol, F.A.W. Coumans, A. Sturk, R. Nieuwland, and T.G. van Leeuwen
Refractive index determination of single extracellular vesicles
LaserLaB symposium 2015
December 10, 2015, Amsterdam, The Netherlands. Invited
24 E. van der Pol
Shining light on individual lipoproteins and microvesicles in plasma
Cardiovascular lecture series, University of Birmingham
November 10, 2015, Birmingham, United Kingdom. Invited
23 E. van der Pol
Dutch physics to elucidate detection of bio nanoparticles from body fluids
DutchBiophysics 2015
September 28, 2015, Veldhoven, The Netherlands. Awarded
22 E. van der Pol
Exosomes enlightened
Photonics Event 2015
June 4, 2015, Veldhoven, The Netherlands. Invited
21 E. van der Pol
Detection of extracellular vesicles: size does matter
Workshop Metrological Characterization of Microvesicles
May 28, 2015, Delft, The Netherlands. Invited
20 E. van der Pol, R. Nieuwland, A. Sturk, T.G. van Leeuwen, and F.A.W. Coumans
Quantitative light scattering of extracellular vesicles for flow cytometry standardization
International Society for Extracellular Vesicles 2015
April 23, 2015, Washington D.C., United States. Awarded
19 E. van der Pol, F.A.W. Coumans, J. Wilke, C. Earhart, B. DiPaolo, R. Hart, B. Cordovez, A. Sturk, R. Nieuwland, and T.G. van Leeuwen
Trapping of single extracellular vesicles in the evanescent field of an optical cavity
SPIE Photonics West 2015
February 9, 2015, San Francisco, United States
18 E. van der Pol, F.A.W. Coumans, A.N. Böing, A. Sturk, R. Nieuwland, T.G. van Leeuwen
Novel techniques for refractive index determination of single nanoparticles in suspension
SPIE Photonics West 2015
February 8, 2015, San Francisco, United States
pdf (1,202 kB)

2014

17 E. van der Pol
Measurements, applications, and impact of the refractive index of extracellular vesicles
Scientific and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis
June 26, 2014, Milwaukee, United States. Invited
pdf (1,452 kB)
16 E. van der Pol, F.A.W. Coumans, Y. Yuana, A. Sturk, T.G. van Leeuwen, R. Nieuwland
Standardization of vesicle detection by flow cytometry using traceable beads and optical scattering theory
International Society for Extracellular Vesicles 2014
May 2, 2014, Rotterdam, The Netherlands
15 E. van der Pol, F.A.W. Coumans, A.N. Böing, A. Sturk, T.G. van Leeuwen, R. Nieuwland
Refractive index of extracellular vesicles by nanoparticle tracking analysis
International Society for Extracellular Vesicles 2014
April 30, 2014, Rotterdam, The Netherlands
pdf (873 kB)
14 E. van der Pol, F.A.W. Coumans, C.M. Hau, C. Otto, A.T.M. Lenferink, A. Sturk, R. Nieuwland, T.G. van Leeuwen
Distinction of tumor-derived vesicles from normal vesicles by Raman microspectroscopy
SPIE Photonics West 2014
February 1, 2014, San Francisco, United States
13 E. van der Pol, F.A.W. Coumans, A.N. Böing, A. Sturk, R. Nieuwland, T.G. van Leeuwen
Refractive index determination of single sub micrometer vesicles in suspension using dark-field microscopy
SPIE Photonics West 2014
February 1, 2014, San Francisco, United States
pdf (1,229 kB)

2013

12 E. van der Pol, C. Gardiner, P. Harrison, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Physical interpretation of the size and concentration of extracellular vesicles
XXIV Congress of the International Society on Thrombosis and Haemostasis
July 2, 2013, Amsterdam, The Netherlands
pdf (662 kB)
11 E. van der Pol
Determination of the refractive index of vesicles using nanoparticle tracking analysis
Scientific and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis
June 29, 2013, Amsterdam, The Netherlands. Invited
pdf (504 kB)
10 E. van der Pol
Detection of microparticles by flow cytometry
“Microparticles: Biomarkers of Disease?” scientific meeting
May 21, 2013, Leicester, United Kingdom. Invited
pdf (1,642 kB)
09 E. van der Pol, C.M. Hau, A.T.M. Lenferink, A. Sturk, R. Nieuwland, C. Otto, and T.G. van Leeuwen
Label-free identification of single cell-derived vesicles by Raman microspectroscopy
SPIE Photonics West 2013
February 6, 2013, San Francisco, United States
08 E. van der Pol, M.J.C. van Gemert, A. Sturk, R. Nieuwland, and T.G. van Leeuwen
Single versus coincidence detection of cell-derived vesicles by flow cytometry
SPIE Photonics West 2013
February 3, 2013, San Francisco, United States
pdf (1,859 kB)

2012

07 E. van der Pol, C. Otto, R. Nieuwland, A. Sturk, and T.G. van Leeuwen
Optical detection and characterization of extracellular vesicles
LaserLaB symposium 2012
December 7, 2012, Amsterdam, The Netherlands. Invited
06 E. van der Pol, R. Nieuwland, and T.G. van Leeuwen
Detecting size, concentration, and chemical composition of extracellular vesicles
Izon Science Nano- and Micro-particle Research Symposium 2012
October 17, 2012, Oxford, United Kingdom. Invited
05 E. van der Pol and R. Nieuwland
Development of reference materials to standardize microvesicle detection
Scientific and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis
June 29, 2012, Liverpool, United Kingdom
pdf (1,564 kB)
04 E. van der Pol, C.M. Hau, C. Otto, A.T.M. Lenferink, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Label-free identification of extracellular vesicles by Raman microspectroscopy
International Society for Extracellular Vesicles 2012
April 19, 2012, Gothenburg, Sweden

2011

03 E. van der Pol, C.M. Hau, C. Otto, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Label-free distinction between platelet and erythrocyte microparticles by Raman microspectroscopy
XXIII Congress of the International Society on Thrombosis and Haemostasis
July 27, 2011, Kyoto, Japan. Awarded
Abstract

Background: The cellular origin of microparticles is usually established by fluorescent antibody labeling, which is laborious, expensive, and involves practical problems (JTH 2010, 8: 2596–607). Using an ultra-modern Raman microspectroscopy setup, we have distinguished platelet from erythrocyte microparticles without labeling.

Methods: Platelet and erythrocyte microparticles were isolated from blood bank concentrates by differential centrifugation and analyzed by flow cytometry, transmission electron microscopy, and Raman microspectroscopy. For Raman microspectroscopy, a 35 mW krypton ion laser operating at a wavelength of 647 nm was focused to a probe volume smaller than 1 μm3 by an Olympus microscope objective with an NA of 0.95. The Stokes shift from light scattered by microparticles diffusing through the probe volume was measured by a sensitive spectrograph dispersing in the range 646–849 nm.

Results: The figure shows that platelet microparticles have a Raman spectrum containing spectral transitions characteristic of phospholipids. The Raman spectrum of erythrocyte microparticles, however, is entirely different.

Conclusions: For the first time, platelet microparticles are distinguished from erythrocyte microparticles without fluorescent antibody labeling.

02 E. van der Pol, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Novel methods to detect microparticles (pdf, 854 kB)
Scientific and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis
July 23, 2011, Kyoto, Japan. Invited
Abstract

We have studied the ability of two new, advanced methods (nanoparticle tracking analysis and resistive pulse sensing with a pore diameter of 1 μm), to measure the size and concentration of a standard population of human urine vesicles by comparison with two established techniques (transmission electron microscopy and flow cytometry). Nanoparticle tracking analysis and resistive pulse sensing detected a concentration of 1.4 × 1010 ml-1 and 3 × 1010 ml-1, respectively, which is 1,000-fold higher than the observed concentration with flow cytometry. By describing the data with a model based on Mie theory, we have obtained the relation between the measured light scattering signal, the refractive index, and the diameter of beads and vesicles. In addition, we provided evidence that the detection of vesicles by flow cytometry is partly attributed to the simultaneous presence of multiple vesicles in the laser beam.

2010

01 E. van der Pol, A.G. Hoekstra, A. Sturk, C. Otto, T.G. van Leeuwen, and R. Nieuwland
An overview of novel and conventional methods to detect microparticles and exosomes (pdf, 825 kB)
Micro and Nanovesicles in Health and Disease
September 23, 2010, Oxford, United Kingdom. Invited