Novel methods to detect microparticles

Oral presentation
E. van der Pol, A. Sturk, T.G. van Leeuwen, and R. Nieuwland
Scienti c and Standardization Committee meeting of the International Society on Thrombosis and Haemostasis, Kyoto, Japan
July 23, 2011
Kyoto 2011 Detect microparticles.pdf (908 kB)


We have studied the ability of two new, advanced methods (nanoparticle tracking analysis and resistive pulse sensing with a pore diameter of 1 μm), to measure the size and concentration of a standard population of human urine vesicles by comparison with two established techniques (transmission electron microscopy and flow cytometry). Nanoparticle tracking analysis and resistive pulse sensing detected a concentration of 1.4 × 1010 ml-1 and 3 × 1010 ml-1, respectively, which is 1000-fold higher than the observed concentration with flow cytometry. By describing the data with a model based on Mie theory, we have obtained the relation between the measured light scattering signal, the refractive index, and the diameter of beads and vesicles. In addition, we provided evidence that the detection of vesicles by flow cytometry is partly attributed to the simultaneous presence of multiple vesicles in the laser beam.