The pathway to single vesicle detection
||E. van der Pol|
||Gordon Research Conference on Extracellular Vesicles 2016, Sunday River, Maine, United States|
||August 20, 2016|
Because most if not all cells release different types of extracellular vesicles (EV), EV in body fluids are heterogeneous. To identify and study the contribution of all EV populations, scientists need to know the origin and function of each single EV in a sample. Flow-based techniques measuring the optical properties of single EV are either sufficiently fast (detection rate of 105 EV s-1), or sensitive (detection of 30 nm EV), or specific (detection of the cellular origin) to accomplish this challenging task. Merging the strengths of contemporary flow-based techniques and standardization approaches will likely result in a test to reliably determine the concentration of EV populations. Such a test enables the addition of EV concentrations to, for example, hematology reference tables, thereby providing physicians more information about their patients.