The list below contains detection methods which we apply to characterize extracellular vesicles in the Vesicle Observation Center.

Impression of SAXS and AFM
Impression of small angle X-ray scattering (left) and atomic force microscopy (right).

Within the Amsterdam University Medical Centers, we have access to:

  • Confocal microscopy (Olympus IX-81 FV1000, Leica TCS-SP5, Leica SP8, Leica SP5-II)
  • Conventional flow cytometry (BD FACSAriaII, BD FACSCantoII, BD FACSscan, BD Influx, BD LSR Fortessa)
  • Fluorescence lifetime imaging microscopy (Nikon-Ti based, Cairn Quadsplit)
  • Scanning electron microscopy (Philips 525)
  • Transmission electron microscopy (Philips CM10, Tecnai G2 Spirit Bio Twin with tomography)

Via our collaborators, we have access to:

  • Anomalous small angle X-ray scattering
  • Atomic force microscopy
  • Raman microspectroscopy
  • Small angle X-ray scattering
  • X-ray fluorescence

Besides the utilization of existing technology, we also develop novel methods to simultaneously detect the size, concentration, and chemical composition of extracellular vesicles.