Metrological characterisation of micro-vesicles from body fluids as non-invasive diagnostic biomarkers
In collaboration with four European metrology institutes we started a project about the metrological characterisation of extracellular vesicles from body fluids as noninvasive diagnostic biomarkers (MetVes).
Aim
The aim of this project is to develop reliable, comparable and quantitative analysis of extracellular vesicles in biological fluids. The research is divided into four Work Packages (WP). In WP1, methods will be developed for the standardized collection and handling of human body fluids for isolation of vesicles, which will be used as input for the other WP's. Methods, available in metrological institutes, will be explored to measure the size of single vesicles, and the size distribution of total vesicle populations (WP2), chemical composition, morphology and concentration (WP3). Synthetic and biological reference materials will be tested as standards for MV measurements (WP4). Selected reference materials will be analysed by all partners and data will be compared between them. The results of this research will enable partners to perform traceable calibrations of MV measurements.